Protein Labeling Reagents
Biotium Eosin-5-isothiocyanate
Eosin-5-isothiocyanate is a good photosensitizer and can be used to selectively label amines. It has a quantum yield of 0.57 for singlet oxygen generation, which makes it useful for photoconversion of electron-rich materials such as diaminobenzidine (DAB) or fluorescent dyes into highly electron-dense materials for high resolution electron microscopy studies. Additionally, eosin is a good fluorescence acceptor for fluorescein (FAM), dansyl and coumarin dyes in fluorescence resonance energy transfer (FRET). Properties: Ex/Em (pH 9) = 521/544 nm; e = 95,000; Orange red solid soluble in DMF or DMSO; Store at -20°C and protect from light; C 21 H 7 Br 4 NO 5 S; MW: 704.96
Biotium 5-(AND-6)-CARBOXY-X-Rhodamine, SUCCINIMI
5(6)-ROX, SE (full name: 5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester) is an amine-reactive long wavelength rhodamine dye. Properties: Ex/Em (MeOH) = 568/595 nm; Dark red solid soluble in DMSO or DMF; Store at -20°C and protect from light; C 37 H 33 N 3 O 7; MW: 631.7; [114616-32-9].
Biotium Aminooxy-5(6)-TAMRA
Aminooxy-5(6)-ROX reacts with aldehydes or ketones to form a stable hydrazone compound under mild condition. Similar to ARP, aminooxy-5(6)-TAMRA can be used to label abasic sites in damaged DNA. Properties: Ex/Em (MeOH) = 540/565 nm; Dark red solid soluble in DMF or DMSO; Store at -20°C and protect from moisture and light; C 36 H 39 F 6 N 5 O 10; MW: 815.71
Biotium Biotin-XX MTSEA,10mg
MTSEA biotin selectively labels thiols under mild conditions. We offer MTSEA biotin with different linker lengths (Biotin-MTSEA, Biotin-X-MTSEA, and Biotin-XX-MTSEA), all of which allow selective labeling of cell surface thiols. Longer linkers should facilitate interaction between biotin and streptavidin. MTSEA biotin-XX MTSEA Biotincapcap; 2-((6-((6-((biotinoyl)amino)hexanoyl)amino)hexanoyl)amino)ethylmethanethiosulfonate Properties: White solid soluble in DMF or DMSO; Store desiccated at -20¿C; C25H45N5O6S3; MW: 607.7
Lumiprobe CYANINE3 NHS ESTER 5MG
Amino-reactive yellow emitting fluorescent dye for the labeling of proteins/peptides and amino-oligonucleotides. For the labeling of sensitive proteins, use water-soluble sulfo-Cyanine3 NHS ester.